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    SourceForge net imagej plugin ihc profiler
    AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed <t>(ImageJ,</t> <t>IHC</t> Profiler <t>plugin)</t> to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).
    Imagej Plugin Ihc Profiler, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagej plugin ihc profiler/product/SourceForge net
    Average 90 stars, based on 1 article reviews
    imagej plugin ihc profiler - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome"

    Article Title: Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome

    Journal: Blood Advances

    doi: 10.1182/bloodadvances.2020001808

    AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed (ImageJ, IHC Profiler plugin) to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).
    Figure Legend Snippet: AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed (ImageJ, IHC Profiler plugin) to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).

    Techniques Used: Staining, Silver Staining, Control, MANN-WHITNEY



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    imagej plugin ihc profiler  (SourceForge net)
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    SourceForge net imagej plugin ihc profiler
    AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed <t>(ImageJ,</t> <t>IHC</t> Profiler <t>plugin)</t> to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).
    Imagej Plugin Ihc Profiler, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imagej plugin ihc profiler/product/SourceForge net
    Average 90 stars, based on 1 article reviews
    imagej plugin ihc profiler - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed (ImageJ, IHC Profiler plugin) to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).

    Journal: Blood Advances

    Article Title: Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome

    doi: 10.1182/bloodadvances.2020001808

    Figure Lengend Snippet: AML infiltration induces proliferation of bone marrow MSPCs. (A) Exemplary procedure for quantification of CD271. First, tile images were fused into larger images (1). Second, a color deconvolution was performed (ImageJ, IHC Profiler plugin) to obtain separate hematoxylin and DAB images. The hematoxylin image was thresholded to obtain a mask of the total bone region (2). The DAB image (3) was inverted and thresholded to obtain a mask of the positive DAB regions (4). Scale bars, 1 mm. (B) Representative images of bone marrow biopsy samples of non-leukemic donors (controls) and AML patients stained for CD271+ MSPCs (brown) and counterstained with hematoxylin (blue). Scale bars, 400 µm. (C) Quantification of CD271+ bone marrow MSPCs in controls (n = 58) and AML patients (n = 36); CD271+ area is calculated by CD271+ stained area divided by total tissue surface. (D) Left: representative image from bone marrow after silver staining representing reticular fibers. Right: image converted by trainable Weka segmentation showing 3 different classes: red, reticular fiber; green, tissue; purple, background. Scale bars, 50 µm. (E) Representative images of bone marrow biopsy samples from controls and AML patients stained for reticular fibers with silver staining (black fiber) and counterstained with the fast-red nuclear solution. Scale bars, 100 µm. (F) Quantification of reticular fibers in control (n = 19) and AML (n = 37) bone marrow by calculating positive silver stained area divided by total tissue surface (n = 56). Data are shown as mean ± standard error of the mean (SEM). *P < .05; **P < .01 (determined by Mann-Whitney U test).

    Article Snippet: Color deconvolution of the images was performed to obtain separate hematoxylin and DAB images using the ImageJ plugin IHC Profiler ( https://sourceforge.net/projects/ihcprofiler/ ).

    Techniques: Staining, Silver Staining, Control, MANN-WHITNEY